| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Leptospira biflexa | TGCGATCGGAATGCCACA | TCCAATCCACACTGCGGAG | 59.73 | 59.70 | 173 | 137 |
100.00%
|
100.00%
|
| Primer 32 | Leptospira biflexa | TGCCGTGCTTGGTTTTGG | AGTCCCTCTGAGCCAGACA | 59.19 | 59.53 | 201 | 137 |
100.00%
|
100.00%
|
| Primer 33 | Leptospira biflexa | TCTGGGGAATACCGTACGGA | TCACACCAACTCTTCTGCCA | 59.74 | 59.16 | 153 | 135 |
100.00%
|
100.00%
|
| Primer 34 | Leptospira biflexa | ACAGTCCCTTGAACACACCA | GGGCACGTTTTGTCGCAT | 59.08 | 59.36 | 167 | 135 |
100.00%
|
100.00%
|
| Primer 35 | Leptospira biflexa | GGTGACAAAGCCAATACGCTG | CGGCATGTTCGCTGTATGC | 60.14 | 60.01 | 154 | 135 |
100.00%
|
100.00%
|
| Primer 36 | Leptospira biflexa | ACATCGGTCAGCTGTGCA | GAATGGCCAGAGTGGTCCA | 59.26 | 59.31 | 226 | 135 |
100.00%
|
100.00%
|
| Primer 37 | Leptospira biflexa | ATCCCAAGCGACAAGGCTC | TCCACTCAACCGCTACACG | 60.08 | 59.71 | 248 | 132 |
100.00%
|
100.00%
|
| Primer 38 | Leptospira biflexa | GGTTCCGACTGCACCTTCA | TGCCACTGCCCACAATCA | 59.93 | 59.48 | 152 | 132 |
100.00%
|
100.00%
|
| Primer 39 | Leptospira biflexa | TGGGTGCGCTGTTTGTGA | TTGGATCAAGGACCGTGCC | 60.12 | 60.00 | 264 | 132 |
100.00%
|
100.00%
|
| Primer 40 | Leptospira biflexa | TGGGTGCGCTGTTTGTGA | GGATCAAGGACCGTGCCAT | 60.12 | 59.78 | 262 | 132 |
100.00%
|
100.00%
|